Fig. 5.

Detection of rDNA copies in tissue homogenates, showing efficacy of amphotericin B (AmB) treatment (A. fumigatus) and ravuconazole (Ravu) treatment (A. terreus) in an experimental model of invasive pulmonary aspergillosis. Log copies per tissue sample and log CFU/g tissue are shown for real-time PCR assays (pan-Aspergillus [black bars] and A. fumigatus or A. terreus species-specific [white bars] assays) and culture (gray bars), respectively. Utilizing the two-tailed Mann-Whitney t test, there was a statistically significant difference in numbers of copies per tissue extraction between the untreated and treated groups for A. fumigatus (P < 0.0001 for amphotericin B at 2.5 mg/kg of body weight/day and P < 0.0003 for amphotericin B at 5.0 mg/kg/day) but not for A. terreus (P = 0.8988).