Table 5.
Sensitivities of culture and real-time PCR assays for detection of Aspergillus spp. in lung tissue specimens from experimental invasive pulmonary aspergillosis
| Species | n | Treatment statusa | No. (%) of positive specimens |
||
|---|---|---|---|---|---|
| Culture | Real-time PCR assay |
||||
| Pan-Aspergillus | Species specific | ||||
| A. fumigatus | 14 | Untreated | 13 (93) | 14 (100) | 14 (100) |
| A. terreus | 6 | Untreated | 6 (100) | 6 (100) | 6 (100) |
| Total | 20 | Untreated | 19 (95) | 20 (100) | 20 (100) |
| A. fumigatus | 11 | Treated (AMB at 2.5 mg/kg/day) | 3 (27) | 6 (55) | 7 (64) |
| 8 | Treated (AMB at 5.0 mg/kg/day) | 4 (50) | 6 (75) | 5 (63) | |
| A. terreus | 6 | Treated (RVC) | 5 (83) | 6 (100) | 6 (100) |
| Total | 25 | Treated | 12 (48) | 18 (72) | 18 (72) |
a
A. fumigatus-infected rabbits were administered liposomal amphotericin B (AMB) at either 2.5 or 5 mg/kg/day for several days prior to assay (6 to 12 days). A. terreus-infected rabbits were administered ravuconazole (RVC) at 10 mg/kg/day for several days prior to assay (12 days).