. 2011 Dec;49(12):4040–4046. doi: 10.1128/JCM.05053-11

Table 2.

Molecular and phenotypic characteristics of C. difficile isolates

CDI clinical outcome	Strain	Ribotype	TRST	CDT^a	tcdC^b	Toxins at 48 h^c				Spores at 48 h^d
CDI clinical outcome	Strain	Ribotype	TRST	CDT^a	tcdC^b	TcdA		TcdB		Spores at 48 h^d
Complicated	383	27	27	+	+	466	H	616	H	L
	390	27	27	+	+	467	H	559	H	L
	391	27	27	+	+	579**	H	474	H	L
	398	18	19	+	wt	106	L	240	L	L
	403	27	27	+	+	571**	H	423	H	L
Severe	395	27	27	+	+	725***	H	500	H	L
	396	27	27	+	+	171	L	242	L	L
	384	53	3	−	wt	104	L	132	L	H
	392	23	14	−	wt	277	L	217	L	H***
	399	6	2	−	wt	95	L	156	L	H
Mild to moderate	386	27	27	+	+	670**	H	662*	H	L
	388	27	27	+	+	647**	H	551	H	L
	389	27	27	+	+	445	H	474	H	L
	393	27	27	+	+	605**	H	425	H	L
	394	27	27	+	+	503	H	416	H	L
	397	27	27	+	+	447	H	445	H	H
	387	27	27	+	+	425	H	379	H	L
	401	27	27	+	+	352	L	408	H	L
	385	18	19	+	wt	204	L	431	H	H
	400	24	65	−	wt	86	L	76	L	H
	402	6	2	−	wt	187	L	19	L	H***

Presence (+) or absence (−) of the cdtA and cdtB genes, encoding the binary toxin CDT.

wt, wild-type tcdC sequence identical to that of the reference strain VPI 10463; +, presence of the common 18-bp deletion in addition to the 1-bp deletion at nucleotide 117.

Relative amounts of toxins detected by immunodot blotting; numbers indicate the mean density of the spots (arbitrary units [AU]) calculated from three independent blots. Letters indicate low (L) and high (H) toxin production compared to the mean densities for TcdA (387 AU [range, 292 to 483]) and TcdB (373 AU [292 to 455]). Asterisks indicate individual values that are significantly different from mean values for non-R027 isolates: *, = P < 0.05; **, P < 0.01; ***, P < 0.001.

L and H, isolates with spore counts at 48 h (spores/ml) below or above the mean for all isolates (1.4 × 10⁷ spores/ml [range, 8.3 × 10⁶ to 2.0 × 10⁷]), respectively. Asterisks indicate isolates that produced significantly more spores than R027 isolates (***, P < 0.001).