Fig. 2.

Retrospective study of immunoreactivity of the hidden malaria. Patient's immunoreactivity against total parasite protein extracts from the clinical isolate cultured at the schizont stage (Western blot) determined in serum samples obtained during the 20-month period of the patient's illness. In brief, the parasite isolate from the patient was grown until a high level of parasitemia was achieved as previously described (16). Next, aliquots of an extract of total parasite protein were separated by SDS-PAGE, transferred onto a nitrocellulose membrane, and separately incubated with stored patient sera obtained on the dates shown at the top of the figure (day/month/year). A horseradish peroxidase-conjugated anti-human IgG secondary antibody was used to detect patient IgGs reacting against specific P. falciparum proteins. SuperSignal (Pierce) was used as the chemiluminescent substrate for visualization.