Fig. 4.

Subcellular defects in the traf4 mutant and overexpressing embryos. Embryos stained with antibodies against the indicated junction-associated proteins. (A, B) E-cadherin (ECad) localization is unaffected in traf4³ mutant embryos (B) compared to that of wild-type embryos (A). (C to F) Canoe (Cno) shows a slight mislocalization in the mesoderm of traf4³ mutant germ line clone-derived embryos at the onset of gastrulation (D) and at stage 6, when wild-type embryos have formed a furrow (F). (C, E) In wild-type embryos, Canoe localizes apically at both stages. (G) Traf4 localizes apically in the mesoderm. (H) Overexpression of Traf4 in the mesoderm using a twist-Gal4 driver displaces Armadillo (Arm) from the junctions. Scale bar, 20 μm.