Fig. 5.

Distribution of E-cadherin and Armadillo in the mesoderm of gastrulating wild-type embryos. (A) Armadillo (green) relocalizes from its subapical to an apical position at the onset of gastrulation (arrowhead). E-cadherin (red) covers a broader domain (brackets), partly colocalizing with Armadillo (arrow). (B) In apically constricted cells, Armadillo colocalizes with E-cadherin in tight apical spots (arrowhead). A gradient of E-cadherin can be seen over the apical quarter of the membrane (brackets). Low-level staining for Armadillo is observed within that domain (arrow). Magnification, ×60; scale bar, 10 μm. (C) Line profile (1 pixel, 68.9 nm) of fluorescence intensities along the cell membrane for E-cadherin (red) and Armadillo (green) measured in the stage 6a embryo shown in panel B plotted against distance (20 μm, x axis). The upper panels show examples for two individual lines given as percentage values of the brightest point (y axis). In the lower graph, the fluorescent intensity values were averaged (5 lines) and are presented as percentages of the brightest average signal (y axis).