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. 2011 Dec;31(24):5046–5060. doi: 10.1128/MCB.05830-11

Fig. 3.

Fig. 3.

Cbp-deficient BM cells are capable of BM reconstitution with reduced efficiency. (A) Noncompetitive transplantation. Representative FACS plots of PB cells from recipients transplanted with either Cbp wt or Cbp Mx cells (left panel). The right panel shows the contributions of Cbp wt and Mx cells to the recipient PB cells over 16 weeks following transplantation. Results are means ± standard errors of the means (SEM; n = 10 for each genotype). (B) Graph demonstrating the percentages of donor-derived B-cell (B220+), T-cell (CD3+), and granulocytic (Mac-1+ Gr-1+) lineages in the PB of recipients at 16 weeks after the noncompetitive transplantation shown in panel A. (C) Cbp-deficient BM cells show no obvious defect in homing. Representative FACS plots are shown for BM cells from recipients transplanted with either Cbp wt or Cbp Mx BM cells that were labeled with CFSE prior to transplantation. The graphs on the right show percentages of total CFSE+, CFSE+ progenitor, and CFSE+ LSK cells of two genotypes detected in the recipient BM cells 24 h after transplantation. Data are means ± SEM (n = 6 for Cbp wt; n = 8 for Cbp Mx). (D) Competitive transplantation. Representative FACS analysis of PB cells from recipients transplanted with Cbp wt and Mx (test) and competitor cells at a 3:1 ratio (left panel). Graphs show the contributions of Cbp wt and Mx cells to the recipient PB reconstitution over a 22-week period following transplantation at a 1:1 ratio (middle panel) and 3:1 ratio (right panel). Results are means ± SEM (n = 5 for each genotype). *, P < 0.05; **, P < 0.01. (E) Deletion of Cbp in the BM niche does not significantly affect the reconstitution ability of wt donor BM cells. The graph shows the contributions of wt donor cells (45.1) to the PB cells of Cbp wt or Cbp Mx recipients over 20 weeks after transplantation (left panel). The right panel shows the percentages of different lineages derived from donor cells in the PB of these recipients at 20 weeks posttransplantation. Results are means ± SEM (n = 5 for each genotype).