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. 2011 Dec;77(23):8259–8264. doi: 10.1128/AEM.05604-11

Fig. 1.

Fig. 1.

(A) PCR amplifications of subA in B173 (VTEC O113 strain isolated from cattle) and C137 (VTEC O146:H21 strain isolated from sheep) using the primer pair SubHCDF/SubSCDR (lanes 2 and 3, respectively) and of subAB in the same strains using the primer pair RTsubABF/RTsubABR (lanes 4 and 5, respectively). Equal amounts of bacterial DNA template were used in each PCR amplification as is shown by the internal 16S rRNA amplification controls in lanes 2 and 3 (100 bp). Lane 1, molecular size marker X (0.07 to 12.2 kbp; Roche, Mannheim, Germany). B173 showed 99.9% homology at the nucleotide level and 100% at the amino acid level with the prototype strain 98NK2. The subA gene in C137 showed 99.4% homology at the nucleotide level and 99.8% at the amino acid level with the prototype strain ED 591. (B) Alignment of primers used in PCR loaded in lanes 2 and 3 and counterpart sequence from subA of the two reference type strains. The nucleotide mismatches according to the PCR primers used for the amplifications are underlined.