Fig. 3.

HAV persistence after transfer of contaminated hemocytes to naïve oysters. The presence of HAV RNA within whole oyster meat was determined by GPTT RNA extraction and RT-PCR. Lanes: 1, 100-bp DNA ladder; 2, uncontaminated oyster prior to hemocyte transfer; 3, whole-oyster sample 1 week after transfer of hemocytes; 4, whole-oyster sample at 2 weeks posttransfer; 5, whole-oyster sample at 3 weeks posttransfer; 6, a subsample of contaminated hemocytes before injection; 7, positive-control mix for RT-PCR, containing 1 μl of HAV, 1 μl of RNase inhibitor, and 8 μl of water; 8, negative-control mix for RT-PCR, containing 10 μl of water.