Fig. 4.
Comparison of extraction methods by use of seeded oyster extracts. Extraction methods were compared using real-time RT-PCR analysis of RNA extracts from hemocyte and whole-tissue fractions of oysters seeded with 10−2 to 104 PFU of hepatitis A virus (A) and murine norovirus (B). Data are reported as recovered PFU equivalents of viral RNA. Statistical differences in recovered PFU for hemocyte-based extraction methods (RNeasy and Dynabeads) relative to the GPTT method were compared using ANOVA. Like letters indicate no significant differences (P > 0.05) between extraction methods.