Table 3.
Real-time RT-PCR detection of viruses in hemocyte and whole-tissue fractions from live oysters exposed to 103, 104, or 105 PFU of HAV or MNV overnight
| Virus and input PFU | Hemocytes |
Whole oysters and GPTT method |
||||
|---|---|---|---|---|---|---|
| Dynabeads |
RNeasy |
|||||
| CT (mean ± SD)a | No. of trials with positive CT/no. of trials | CT (mean ± SD)a | No. of trials with positive CT/no. of trials | CT (mean ± SD)a | No. of trials with positive CT/no. of trials | |
| HAV | ||||||
| 103 | NA | 0/3 | NA | 0/3 | NA | 0/3 |
| 104 | 39.74 | 1/3 | 37.71 | 1/3 | 36.95 | 2/3 |
| 105 | 36.03 ± 0.80 | 3/3 | 36.86 ± 1.73 | 3/3 | 36.73 ± 0.42 | 3/3 |
| MNV | ||||||
| 103 | 37.75 | 2/3 | 36.47 ± 0.98 | 3/3 | 39.01 | 2/3 |
| 104 | 34.65 ± 2.08 | 3/3 | 33.13 ± 2.80 | 3/3 | 35.45 ± 2.92 | 3/3 |
| 105 | 30.33 ± 1.23 | 3/3 | 30.12 ± 1.29 | 3/3 | 33.80 ± 1.39 | 3/3 |
a
Data are means of positive results or individual results if at least 1 of 3 trials was positive. NA, not applicable.