Table 4.
Real-time RT-PCR detection of viruses in hemocyte and whole-tissue fractions from oysters seeded with 104 to 10−4 PFU of HAV and MNV
| Virus and input PFU | Hemocytes |
Whole oysters and GPTT method |
||||
|---|---|---|---|---|---|---|
| Dynabeads |
RNeasy |
|||||
| CT (mean ± SD)a | No. of trials with positive CT/no. of trials | CT (mean ± SD)a | No. of trials with positive CT/no. of trials | CT (mean ± SD)a | No. of trials with positive CT/no. of trials | |
| HAV | ||||||
| 10−2 | NA | 0/3 | NA | 0/3 | NA | 0/3 |
| 10−1 | NA | 0/3 | NA | 0/3 | NA | 0/3 |
| 100 | 39.87 | 1/3 | 39.61 | 1/3 | NA | 0/3 |
| 101 | 38.69 ± 0.72 | 3/3b | 35.72 ± 1.17 | 3/3b | 38.30 | 2/3 |
| 102 | 37.28 ± 0.65 | 3/3 | 33.31 ± 1.66 | 3/3 | 36.40 ± 0.72 | 3/3b |
| 103 | 33.39 ± 0.55 | 3/3 | 29.52 ± 1.01 | 3/3 | 32.62 ± 0.37 | 3/3 |
| 104 | 29.71 ± 0.44 | 3/3 | 26.07 ± 0.82 | 3/3 | 28.96 ± 1.15 | 3/3 |
| MNV | ||||||
| 10−2 | 33.56 | 1/3 | 37.31 | 2/3 | 39.66 | 2/3 |
| 10−1 | 35.79 | 2/3 | 35.93 ± 1.18 | 3/3b | 38.71 | 1/3 |
| 100 | 35.75 ± 2.78 | 3/3b | 33.02 ± 0.50 | 3/3 | 37.32 | 1/3 |
| 101 | 33.00 ± 1.30 | 3/3 | 29.71 ± 0.86 | 3/3 | 36.01 ± 0.93 | 3/3b |
| 102 | 30.53 ± 0.58 | 3/3 | 25.67 ± 2.22 | 3/3 | 33.46 ± 2.80 | 3/3 |
| 103 | 27.99 ± 0.80 | 3/3 | 22.59 ± 1.25 | 3/3 | 30.15 ± 1.84 | 3/3 |
| 104 | 24.11 ± 0.44 | 3/3 | 18.59 ± 1.06 | 3/3 | 25.93 ± 2.00 | 3/3 |
a
Data are means of positive results or individual results if at least 1 of 3 trials was positive. NA, not applicable.
b
Sample from which LOD was derived.