Table 1.
Characterization of ATDa
| Culture medium | No. of CFU/10 mgb | Organisms (no.) identifiedc |
|---|---|---|
| R2A | 4.4 × 104 ± 2.1 × 103 | Yeast (1), Micrococcus luteus (1) |
| TSAB | 5.1 × 104 ± 9.6 × 103 | B. licheniformis (4), B. cereus (5), Micrococcus roseus (2) |
| Sabouraud dextrose agar | 7.6 × 103 ± 1.3 × 103 | B. cereus (4), B. licheniformis (3), B. mycoides (2), B. endophyticus (1), actinomycetes (9), yeast (2), Streptomyces sp. (1), molds (4) |
a
ATD (Powder Technology Inc., Burnsville, MN) was suspended in PBS plus 0.02% Tween 80 to a final concentration of 10 mg/ml and cultured on the media indicated to establish the composition of the existing flora.
b
Triplicate dilutions, triplicate plating, n = 9.
c
Thirty-nine morphologically distinct colony types were found during dilution plating on TSAB, R2A, and Sabouraud dextrose agar. Standard morphological testing were used to determine identity.