Table 1.
Primers used to amplify virulence-associated genes actA, prfA, inlA, inlB, inlC, inlD, inlE, inlF, inlG, inlJ, and inlC2H
| Primer use and gene or primer name | Sequence (5′–3′) |
|
|---|---|---|
| Forward primer | Reverse primer | |
| Determination of gene size variations | ||
| actA | GTTGATAAAAGTGCAGGGTTAATTG | GCAATTAGTTTTCCTTCTTCAATGC |
| prfA | ATGAACGCTCAAGCAGAAGAATTC | TTAATTTAATTTTCCCCAAGTAGCA |
| inlA-a | TAACAAAGCTTCAAAGATTATTTTTCTATAATAAC | TCAGTCAATAAATTCCCAGCTTCC |
| inlA-b | AAGCTGGGAATTTATTGACTGAACC | TGAATTATAAGGGTCATAAGCGTTCATT |
| inlB | TGGTTTTCGGACTATATCTAGCTTTT | TAAAATCGTTTCCGGACATATAATCC |
| inlC | TGCTAACATATAAGTATACAAAGGGACA | AACTGTTCCATCAAATATAGCCTCA |
| inlD | AACTCTGGCTGTAGTAATGGCAAT | CGCCAGTTTTCGCATCATAC |
| inlE | TAAATTCATCTAAACGTCACAACTAAA | TGACTGATTGGTTAAATGTGTAACTAA |
| inlF | AGAAGCGGAAATTTGCATATTAA | ACCAACCATCAAATGTATAACCTTG |
| inlG | AAACCTCAGTACTACATGTTTTACTTGTAG | TCCTACAAATGTGTAGCCATCTTTC |
| inlJ | GACATCCCAGATTATACATTAACGACTACT | TTTGCCATTAGCATCCACGTAATTCA |
| inlC2H | GTTTTAATGGTAACTGCTATTCTCGG | AAAACCGCAGTGTAAGCTTCTG |
| Amplification of actA and inlJ | ||
| inlJ-a | AGTCATTAAACCGACACAATCGAGT | CAGTCTAGCTCAGTTAATTGTGTATTGTGG |
| inlJ-b | AACTTATTTAAACTGCGCACGCA | TTAAAGTTTCTTTAGGCATCGTGATTG |
| inlJ-c | TTGCGTAAATGCTCACATCCAA | TGTACACGTACGTTACGGACTGGC |
| inlJ | GACATCCCAGATTATACATTAACGACTACT | TTTGCCATTAGCATCCACGTAATTCA |
| inlJ-d | GCTAACAACTACACCAGATAACGCAACC | ACTGGATGTGGGTGTTTCTATCCAAGT |
| actA-a | TTTCCTTGTTCTAAAAAGGTTGTATTAGC | GTGGAAAGTCCGAAGCATTTACC |
| actA | GTTGATAAAAGTGCAGGGTTAATTG | GCAATTAGTTTTCCTTCTTCAATGC |
| actA-b | TGAAACCGCAAACCGAGG | ATTTACCTCAGTATTTGTCGGATTATCC |