Fig. 1.
Effects of temperature and pH on pressure inactivation of HRV in aqueous medium. (A) The effect of temperature on HRV inactivation. HRV stock (106 PFU/ml) in cell culture medium (MEM) was processed under pressures ranging from 200 MPa to 450 MPa held for 2 min at either 4°C or 20°C. (B) The effect of pH on HRV inactivation. HRV stock (105.5 PFU/ml) in MEM was adjusted to pH 4.0 or 7.0 using citric acid. Samples were subjected to pressure treatment at 250 MPa and 300 MPa held for 2 min at 4°C. The surviving viruses were determined by plaque assay. Data are the means of three replicates. Error bars represent standard deviations.