Fig. 8.

The effect of HPP on viral genomic RNA. (A) The effect of HPP on VSV genomic RNA. Purified VSV was treated at 550 MPa and 20°C for 8 min and 10 min, respectively. The samples were digested with 10 units of RNase at 37°C for 1 h. Viral genomic RNA was extracted from either HPP-treated or untreated VSV. The N gene of VSV was amplified by one-step RT-PCR, and PCR products were visualized by 1% agarose gel electrophoresis. (B) The effect of HPP on HRV genomic RNA. Purified HRV TLPs were treated at 450 MPa at 4°C for 2 min, followed by RNase digestion and RNA extraction. The VP7 gene of HRV was amplified by one-step RT-PCR.