Table 2.
Immersion of O. hermsi larvae in a mixed suspension and nymphal transmission to mice
| B. hermsii isolate used for immersion | Tick group | No. of ticks positive by IFA/total no. of ticks fed | Presence of mouse spirochetemia | Mean gene copy no. ± SDa |
||
|---|---|---|---|---|---|---|
| rrs | vtp-6 | vtp-5 | ||||
| DAH | 1 | 0/3 | − | 0 | 0 | 0 |
| 2 | 0/1 | − | 0 | 0 | 0 | |
| MTW-2 | 1 | 0/3 | − | 0 | 0 | 0 |
| 2 | 1/3 | + | 950.4 ± 11.7 | 0 | 921.2 ± 129.5 | |
| DAH + MTW-2 | 1 | 1/3 | + | 5,822.3 ± 417.8 | 2.4 ± 0.8 | 3,333.4 ± 241.2 |
| 2 | 0/3 | − | 0 | 0 | 0 | |
| 3 | 1/2 | + | 1,416.7 ± 133.3 | 0 | 465.9 ± 134.7 | |
| 4 | 0/2 | − | 0 | 0 | 0 | |
| 5 | 2/3 | + | 421.8 ± 56.6 | 6.4 ± 0.6 | 60.4 ± 11.4 | |
| 6 | 1/2 | + | 2,305.7 ± 27.3 | 991.5 ± 150.6 | 794.3 ± 22.5 | |
a
Determined by qPCR of mouse blood. The assay represents gene copies per 2.5 ng total nucleic acid.