Fig. 3.

(a) Transformations of NO₃⁻ and Fe(II)-EDTA by Acidovorax sp. 2AN in batch cultures. Medium C contained only 5 mM Fe(II)-EDTA as the electron donor. Medium M contained both 5 mM Fe(II)-EDTA and 1.2 mM acetate. All media and controls contained 5 to 6 mM NO₃⁻. The data for heat-killed controls are presented in the supplemental material. The dotted and dashed lines, respectively, represent points when 7 mM nitrate and 4 mM Fe(II)-EDTA were re-added. (b) Consumption of acetate and accumulation of extracellular nitrite in the medium. The dashed lines represent the points when ca. 1 mM acetate was re-added. (c) Growth of strain 2AN in medium C and medium M. The data are presented as mean ± the standard deviations (n = 3). When not shown, error bars are smaller than the symbol size.