Table 3.

rarA primer sequences^a

PCR amplification site	Primer		Locationb	Amplicon size (bp)
	Forward	Reverse
Intact rarA gene	ATGAGGGTACTTGTATCCTCC	GTTGCAGAAGATATTGGTTTG	1169159–1168918	241
Intact rarA gene + flanking	CAATGCTCTCTCGAATAGAAA	GTTGCAGAAGATATTGGTTTG	1169460–1168918	542
5′ rarA gene + flanking	GATCCTCACATCTTCCTTCTC	TCCTTTAGCTGATTTGATGC	1161570–1161092	478
5′ rarA gene	ATCTTTTACCGATGCTGTTG	TCCTTTAGCTGATTTGATGC	1161282–1161092	190
3′ rarA gene + flanking	ACATAATGAATATGGCAGGC	CCTTGTGTAAAGTCATTTGTGA	1185696–1185103	593
3′ rarA gene	TGGAAGAATAACGTTAATAGCA	CCTTGTGTAAAGTCATTTGTGA	1185696–1185458	238

^ararA primer sequences and characteristics. Sequences for six PCR primer pairs that amplify intact rarA, regions within the 5′ or 3′ split rarA gene, and/or its flanking region. The primer sites within C. botulinum Alaska E43 and the expected amplicon sizes are provided.

^bLocation within C. botulinum Alaska E43 (NC_010723.1).