Table 4.
VNTR primer sequences and characteristicsa
| VNTR | Primer sequence | Genomic locationb | Sequence of repeat unit | Repeat unit size (bp) | Diversity index |
|---|---|---|---|---|---|
| 21F | TGCATTCAGGACATAACTCTT | 1021136–1021489 | TTCTTATC | 8 | 0.8959 |
| 21R | TATGTTTCCGATAAGTGTTCAT | ||||
| 24F | TGGAATGAGTGGAAGAAGTG | 1966109–1966453 | ATTTTAATTAAAAGGAG | 17 | 0.2998 |
| 24R | ATGCTTGCCATATTGAAATC | ||||
| 25F | TAACAGTCGTAGGGTGTTAGC | 2468172–2468657 | AGAATTTA | 8 | 0.9328 |
| 25R | TGGAACTCTATGTGCTGAAAG |
a
VNTR primer sequences and characteristics. Shown are forward (F) and reverse (R) primer sequences to amplify and/or sequence the three VNTR regions identified in the serotype E strains. Values for the repeat unit, size, and diversity index are provided.
b
Location within C. botulinum Alaska E43 (NC_010723.1).