Table 5.
Nucleotide and amino acid identities among BoNT/E subtypes by domain
| BoNT/E subtype comparison | % Nucleotide/% amino acid similarities ina: |
|||
|---|---|---|---|---|
| LC (bp 1-1260) (aa 1-420) | Hn (bp 1261-2499) (aa 421-833) | Hc (bp 2500-3756) (aa 834-1252) | HT (bp 1-3756) (aa 1-1252) | |
| E1 vs: | ||||
| E2 | 99.9/99.8 | 100/100 | 98.3/97.6 | 99.4/99.1 |
| E3 | 97.9/94.8 | 100/100 | 100/100 | 99.3/98.2 |
| E4 | 97.9/96.0 | 99.0/98.3 | 98.8/97.9 | 98.5/97.4 |
| E5 | 100/100 | 98.5/97.8 | 96.3/92.8 | 98.3/96.9 |
| E6 | 98.7/96.7 | 98.1/97.1 | 98.6/97.1 | 98.5/97.0 |
| E7 | 97.4/94.0 | 99.8/99.8 | 100/100 | 99.1/97.8 |
| E8 | 97.4/94.0 | 98.4/97.3 | 97.9/97.4 | 97.9/96.2 |
| E2 vs: | ||||
| E3 | 97.9/94.5 | 100/100 | 98.3/97.6 | 98.7/97.4 |
| E4 | 97.8/95.7 | 99.0/98.3 | 98.3/97.4 | 98.3/97.1 |
| E5 | 99.9/99.8 | 98.5/97.8 | 95.5/91.4 | 97.9/96.3 |
| E6 | 98.6/96.4 | 98.1/97.1 | 98.1/96.9 | 98.2/96.8 |
| E7 | 97.4/94.0 | 99.8/99.8 | 98.3/97.4 | 98.5/97.0 |
| E8 | 97.4/94.0 | 98.4/97.3 | 99.6/99.8 | 98.5/97.0 |
| E3 vs: | ||||
| E4 | 95.9/91.0 | 99.0/98.3 | 98.8/97.9 | 97.9/95.7 |
| E5 | 97.9/94.8 | 98.5/97.8 | 96.3/92.8 | 97.6/95.1 |
| E6 | 97.2/93.3 | 98.1/97.1 | 98.6/97.1 | 98.0/95.8 |
| E7 | 96.5/92.4 | 99.8/99.8 | 100/100 | 98.8/97.3 |
| E8 | 96.5/92.4 | 98.4/97.3 | 97.9/97.4 | 97.6/95.7 |
| E4 vs: | ||||
| E5 | 97.9/96.0 | 98.5/98.1 | 95.4/90.9 | 97.2/95.0 |
| E6 | 96.8/93.6 | 98.4/97.8 | 99.8/99.3 | 98.3/96.9 |
| E7 | 96.8/92.9 | 98.8/98.1 | 98.8/97.6 | 98.1/96.2 |
| E8 | 96.8/92.9 | 98.8/98.1 | 98.3/97.6 | 98.0/96.2 |
| E5 vs: | ||||
| E6 | 98.7/96.7 | 97.7/96.9 | 95.3/90.7 | 97.2/94.7 |
| E7 | 97.4/94.0 | 98.4/97.6 | 96.3/92.6 | 97.3/94.7 |
| E8 | 97.4/94.0 | 97.9/97.1 | 95.5/91.2 | 96.9/94.1 |
| E6 vs: | ||||
| E7 | 97.9/95.0 | 98.1/96.9 | 98.6/96.9 | 98.2/96.2 |
| E8 | 97.9/95.0 | 98.9/98.3 | 98.1/97.1 | 98.3/96.8 |
| E7 vs: | ||||
| E8 | 100/100 | 98.5/97.6 | 97.9/97.4 | 98.8/98.3 |
a
Percent nucleotide and amino acid identities among BoNT/E subtypes by domain. Nucleotide and amino acid comparisons (presented % nucleotide/% amino acid) of eight strains representing the bont/E1 to E8 subtypes were performed to identify the percent similarities between the subtypes in the light chain (LC), translocation domain (Hn), receptor-binding domain (Hc), and holotoxin (HT). The greatest variation observed in the holotoxin is 3.1% (nucleotide) or 5.9% (amino acid) with E5 compared to E8. Strains selected for the comparisons include (E1) E213/Beluga, (E2) CDC5247, (E3) Alaska E43, (E4) BL5262, (E5) LCL155, (E6) K36, (E7) IBCA97-0192, and (E8) NYDH Bac-02-06430.