Table 1.
qPCR assay specificity check for Planktothrix rubescens based on Cq values obtained for mixtures of target and nontarget species DNAs
| Organism(s)a | Strain(s) | Ratio of mixture |
Cq value (mean ± SD) |
||||
|---|---|---|---|---|---|---|---|
| TNA-PlPC | TNA-16S rRNA | TNA-MAP with dilution factor of: |
|||||
| 1 | 0.1 | 0.01 | |||||
| P. rubescens | TCC 29 | 21.52 ± 0.04 | 19.57 ± 0.05 | 16.82 ± 0.07 | 20.58 ± 0.11 | 24.83 ± 0.08 | |
| P. rubescens and | TCC 29/HUB 524 | 1:1 | 21.74 ± 0.07 | 19.61 ± 0.03 | |||
| Microcystis | TCC 29/HUB 524 | 1:100 | 21.94 ± 0.03 | 19.89 ± 0.19 | |||
| Microcystis | HUB 524 | 33.11 ± 0.04 | 33.82 ± 0.07 | ||||
| P. rubescens and | TCC 29/TCC 3a | 1:1 | 21.95 ± 0.13 | 20.15 ± 0.04 | |||
| Lyngbya | TCC 29/TCC 3a | 1:100 | 21.81 ± 0.03 | 19.98 ± 0.08 | |||
| Lyngbya | TCC 3a | 34.12 ± 0.07 | 33.91 ± 0.03 | ||||
| P. rubescens and | TCC 29/TCC 32 | 1:1 | 21.78 ± 0.02 | 19.58 ± 0.08 | 16.68 ± 0.06 | 20.12 ± 0.04 | 24.89 ± 0.02 |
| Synechococcus | TCC 29/TCC 32 | 1:10 | 16.97 ± 0.05 | 20.59 ± 0.08 | 24.53 ± 0.23 | ||
| TCC 29/TCC 32 | 1:100 | 22.03 ± 0.02 | 19.95 ± 0.09 | 16.74 ± 0.05 | 20.22 ± 0.05 | 24.78 ± 0.03 | |
| Synechococcus | TCC 32 | 32.17 ± 0.15 | 32.27 ± 0.07 | 31.72 ± 0.22 | —b | —b | |
| P. rubescens and | TCC 29/TCC 79 | 1:1 | 22.32 ± 0.12 | 20.14 ± 0.09 | |||
| Anabaena | TCC 29/TCC 79 | 1:100 | 21.84 ± 0.06 | 19.96 ± 0.12 | |||
| Anabaena | TCC 79 | 32.59 ± 0.04 | 32.56 ± 0.05 | ||||
a
Mixes of P. rubescens strain TCC 29 and background DNAs originating from other organisms (Microcystis strain HUB 524, Lyngbya strain TCC 3a, Synechococcus strain TCC 32, and Anabaena strain TCC 79).
b
—, below the detection limit.