Table 2.
Target genes and primer sequences used for the detection of STEC virulence gene markers
| Primer | Sequence (5′–3′) | Target (gene) | Reference |
|---|---|---|---|
| stx1-F | ATAAATCGCCATTCGTTGACTAC | stxA1 | 54 |
| stx1-R | AGAACGCCCACTGAGATCATC | ||
| stx2-F | GGCACTGTCTGAAACTGCTCC | stxA2 | 54 |
| stx2-R | TCGCCAGTTATCTGACATTCTG | ||
| eaeA-F | GACCCGGCACAAGCATAAGC | eaeA | 54 |
| eaeA-R | CCACCTGCAGCAACAAGAGG | ||
| hlyA-F | GCATCATCAAGCGTACGTTCC | hlyA | 54 |
| hlyA-R | AATGAGCCAAGCTGGTTAAGCT | ||
| TIR-F | CATTACCTTCACAAACCGAC | tir | 40 |
| TIR-R | CCCCGTTAATCCTCCCAT | ||
| EspAa | CACGTCTTGAGGAAGTTTGG | espA | 48 |
| EspAb | CCGTTGTTAATGTGAGTGCG | ||
| EspBa | CGATGGTTAATTCCGCTTCG | espB | 48 |
| EspBb | CGATGGTTAATTCCGCTTCG | ||
| ESPPa | AAACAGCAGGCACTTGAACG | espP | 48 |
| ESPPb | AGACAGTTCCAGCGACAACC | ||
| D1 | CGTCAGGAGGATGTTCAG | etpD | 65 |
| D13R | CGACTGCACCTGTTCCTGATTA | ||
| wkat-B | CTTCCTGTTCTGATTCTTCTGG | katP | 11 |
| wkat-F | AACTTATTTCTCGCATCATCC | ||
| lpfO141-F | CTGCGCATTGCCGTAAC | lpfAO157/OI-141 | 69 |
| lpfO141-R | ATTTACAGGCGAGATCGTG | ||
| lpfA-F | ATGAAGCGTAATATTATAG | lpfAO113 | 16 |
| lpfA-R | TTATTTCTTATATTCGAC | ||
| O154-FCT | GCAGGTCACCTACAGGCGGC | lpfAO157/OI-154 | 71 |
| O154-RCT | CTGCGAGTCGGCGTTAGCTG | ||
| SAADF | CGTGATGAACAGGCTATTGC | saa | 55 |
| SAADR | ATGGACATGCCTGTGGCAAC | ||
| toxB.911F | ATACCTACCTGCTCTGGATTGA | toxB | 70 |
| toxB.1468R | TTCTTACCTGATCTGATGCAGC | ||
| iha-I | CAGTTCAGTTTCGCATTCACC | iha | 68 |
| iha-II | GTATGGCTCTGATGCGATG | ||
| LH0147-BamHI | CCCGGATCCGGAAACTCCAAGAGTATTGC | sab | 33 |
| LH0147-EcoRI | CCCGAATTCCCTTGCTTTTCCCTGTTACC | ||
| KS7 | CCCGGATCCATGAAAAAAACATTATTAATAGC | stx1b | 66 |
| KS8 | CCCGAATTCAGCTATTCTGAGTCAACG | ||
| Stx1c-1 | TTTTCACATGTTACCTTTCCT | stxA1c | 77 |
| Stx1c-2 | CATAGAAGGAAACTCATTAGG | ||
| VT1varF | CTTTTCAGTTAATGCGATTGCT | stxA1d | 12 |
| VT1varR | AACCCCATGATATCGACTGC | ||
| LP43 | ATCCTATTCCCGGGAGTTTACG | stxA2 variants | 13 |
| LP44 | GCGTCATCGTATACACAGGAGC | ||
| GK3 | ATGAAGAAGATGTTTATG | stxB2 and stxB2c | 32 |
| GK4 | TCAGTCATTATTAAACTG | ||
| SLT-II-vc | ACCACTCTGCAACGTGTCGC | stx2c and stx2dact | 36 |
| CKS2 | ACTGAATTGTGACACAGATTA | ||
| VT2-cm | AAGAAGATATTTGTAGCGG | stxB2d | 61 |
| VT2-f | TAAACTGCACTTCAGCAAAT | ||
| FK1 | CCCGGATCCAAGAAGATGTTTATAG | stxB2e | 21 |
| FK2 | CCCGAATTCTCAGTTAAACTTCACC | ||
| 128-1 | AGATTGGGCGTCATTCACTGGTTG | stxA2f | 67 |
| 128-2 | TACTTTAATGGCCGCCCTGTCTCC | ||
| 209F | GTTATATTTCTGTGGATATC | stx2g | 44 |
| 781R | GAATAACCGCTACAGTA | ||
| VSAAF | ACTCGCATAATTGGTGGTG | saa variants | 45 |
| VSAAR | ATCATTGGTATTGCTGTCAT | ||
| EaeVF | AGYATTACTGAGATTAAG | eae variants | 64 |
| EaeVR | AAATTATTYTACACARAY | ||
| EaeZetaVR | AGTTTATTTTACGCAAGT | ||
| EaeIotaVR | TTAAATTATTTTATGCAAAC |