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SDS-PAGE analysis of protein extracts showing linuron hydrolase activity, from linuron-amended Variovorax sp. SRS16 cultures at different purification steps. Lane 1, crude cell-free protein extract; lane 2, after 35 to 50% ammonium sulfate precipitation; lane 3, after anion-exchange chromatography; lane 4, after size exclusion chromatography. Band A was identified as transcription elongation factor G, while band B was identified as the translation product of ORF 42 (libA).
