Table 4.
Time zero quantification by wet weight, viable count, and qPCRa
| Strain | Wet wt |
Viable count |
qPCR |
|||||
|---|---|---|---|---|---|---|---|---|
| (Wet wt0) (bacteria/ml) | (Wet wt0/wet wti) | (VC0) (CFU/ml) | (VC0/wet wti) | (VC0/wet wt0) (GE/ml) | (qPCR0) | (qPCR0/wet wti) | (qPCR0/wet wt0) | |
| 316F | 1.92 × 107 | 112.87 | 1.99 × 105 | 1.17 | 0.01036 | 7.97 × 104 | 0.47 | 0.00415 |
| 2E | 3.33 × 107 | 195.96 | 1.90 × 104 | 0.11 | 0.00057 | 1.13 × 104 | 0.07 | 0.00034 |
| K10 | 2.49 × 107 | 149.69 | 8.93 × 104 | 0.53 | 0.00358 | 1.91 × 104 | 0.11 | 0.00077 |
| 81 | 2.69 × 107 | 158.09 | 8.60 × 104 | 0.51 | 0.00320 | 3.92 × 104 | 0.23 | 0.00146 |
| 445 | 1.74 × 107 | 102.57 | 5.43 × 104 | 0.32 | 0.00311 | 3.19 × 104 | 0.19 | 0.00183 |
| 764 | 2.43 × 107 | 142.65 | 5.33 × 104 | 0.31 | 0.00220 | 4.21 × 104 | 0.25 | 0.00174 |
| 22G | 1.78 × 107 | 104.78 | 3.78 × 104 | 0.22 | 0.00212 | 7.58 × 104 | 0.45 | 0.00425 |
| OC 49 | 1.12 × 107 | 65.81 | ND | ND | ND | 2.88 × 104 | 0.17 | 0.00258 |
Wet wt0, wet weight value at time zero; wet wti, wet weight initial inoculum value; VC0, viable count value at time zero; qPCR0, qPCR value at time zero; ND, not determined. Turbidometry could not be compared because all the values were 0 or below. Wet wti = 1.7 × 105 bacteria/ml.