Fig. 5.

Thermal denaturation studies of equimolar mixtures of the HR1 (N36) and HR2 (C34) peptides containing wild-type residues or mutations, as indicated in Fig. 1. (A) The α-helical conformation of the complex formed by N36-C34 mixtures (10 μM each in phosphate-buffered saline) was analyzed by CD spectroscopy. (B) The fraction of unfolded N36-C34 mixtures was calculated from CD measurements at 222 nm as a function of temperature. Two independent experiments gave identical results. The N36 and C34 mixtures are as indicated.