Table 1.

Emerging mutations in the envelope glycoprotein after the indicated passage

Culture and passagea	gp120 mutation(s)	gp41 mutation(s)b
		HR1	Hinge	HR2	C tail
Cltr 1
P3	L125F
P5	L125F, I165K	E560K
P6	L125F, I165K	E560K, V580L
P7	L125F, I165K	N554K, E560K, V580L		T641I
P11	L125F, I165K	N554K, E560K, A561V, V580L		T641I
Cltr 2
P2	L125F
P4	L125F, E429K
P5	L125F, E429K	E560K
P7	L125F, E429K	E560K, Q577R
P11	L125F, E429K	E560K, Q577R
Cltr 3
P2	T319I
P4	T319I			E648K
P11	T319I			E648K
Cltr 4
P4		Q577R		E648K
P5	S306G, T319I	Q577R		E648K	V833I
P8	S306G, T319I, E322D	Q577R	D620N	E648K	V833I
P14	S306G, T319I, E322D	Q577R	D620N	T641I, E648K	V833I
P15	S306G, T319I, E322D	Q577R	D620N	T641I, E648K	V833I
Con 1
P7	E172G
P11	E172G
Con 2
P5	Q170R
P7	S115R, Q170R
P11	S115R, Q170R
Con 3
P5	F317Y
P11	F317Y
Con 4
P5	D167N
P11	L125F, D167N

^aMutations arising during 11 passages (P0 to P11) of HIV-1 cultures propagated in the presence (cultures 1 to 4) or absence (controls 1 to 4) of the N44 peptide are shown. Culture 4 was a continuation of the culture that we reported previously (16), which acquired the Q577R and E648K mutations after four passages (P4). Culture 4 in this report was selected for another 11 passages (to P15). Cltr, selection culture; Con, control culture; P, passage.

^bHR1, heptad repeat 1; HR2, heptad repeat 2; C-tail, cytoplasmic tail.