Fig. 3.

The carboxy-terminal cargo-binding domain of TNPO3 is required for SIVmac239 infection. (A) The shRNA-target site in TNPO3 (red) was mutated (black) to generate an shRNA-resistant TNPO3 expression vector. (B) TNPO3 knockdown 293T cells were transfected with expression vector encoding shRNA-resistant TNPO3, importin-13 (Imp13), and empty vector. The transfected cells and wild-type 293T cells (cntrl cells) were then infected with SIVmac239 reporter virus. (C) TNPO3 knockdown 293T cells were transfected with wild-type, ΔCargo, or TNPO3 with the carboxy-terminal 18 amino acids deleted (ΔC18).