Fig. 6.

A dileucine motif in the cargo-binding domain of TNPO3 is required to facilitate SIVmac239 infection. (A) Specific amino acid residues in the cargo-binding domain were mutated to alanine (asterisks). Individual loop mutants are boxed in black. (B) TNPO3 knockdown cells were complemented with a wild-type, ΔCargo, or mutated TNPO3 expression vector. (C) TNPO3 knockdown cells were complemented with a wild-type, ΔCargo, and TNPO3 expression vector containing single or multiple point mutations of amino acid residues L767, L766, R769, and S770. (D) The mutated TNPO3 proteins were visualized by immunoblot analysis.