Fig. 5.

Increase in fluorescence of acrylodan-labeled gHtgL after addition of integrin is not a result of protein aggregation. (A) Overlay of emission spectra of unlabeled gHtgL alone and 5 min and 60 min after addition of αvβ8. (B) Overlay of emission spectra of acrylodan-labeled gHtgL measured immediately (1) or after 1 h (2). (C) Time-based emission fluorescence of acrylodan-labeled gHtgL after addition of αvβ8. At the time point indicated by the arrow, the reading was stopped, the sample was centrifuged at 16,000 × g for 15 min, and the sample was returned to the cuvette for resumption of the reading. (D) Overlay of emission spectra of acrylodan-labeled gHtgL alone and 5 min, 60 min, and 120 min after addition of αvβ3. Fluorescence was recorded at an excitation wavelength of 359 nm and an emission wavelength of 380 to 600 nm (A, B, and D) or an excitation wavelength of 359 nm and an emission wavelength of 540 nm (C).