Skip to main content
. 2011 Dec;85(24):13164–13173. doi: 10.1128/JVI.05808-11

Fig. 3.

Fig. 3.

PMLIVa stably expressed in U373MG or CHO cells inhibits EMCV multiplication. (A and B) U373MG-EV cells and U373MG cells stably expressing PMLIVa were infected with EMCV at an MOI of 0.5 for 12 h, and the cells were analyzed by Western blotting for viral proteins, PML, and actin (A) or the viral titers were determined (B). (C and D) Immunofluorescence (red) of PML in CHO-PMLIVa and CHO-EV is shown in the top micrographs. CHO cells containing EV or stably expressing PMLIII or PMLIVa, prepared in duplicate, were similarly infected with EMCV. One series of cells was analyzed by Western blotting for viral antigens and actin expression (bottom panel). The second series was used for the determination of viral titers (D). (E) Fate of PMLIII and PMLIV during EMCV infection. U373MG cells stably expressing PMLIII or PMLIV were infected for 12 h at various MOIs, and cell extracts were then analyzed by Western blotting using anti-PML and antiactin antibodies.