Fig. 1.

Identification of PRRSV nsp1β-interacting proteins by IP-MS. Flag-EV (Vector)- and Flag-nsp1β (nsp1β)-overexpressing 293T cells were used for immunoprecipitation using anti-Flag agarose resins. Eluted proteins were resolved by 12% SDS-PAGE and subjected to silver staining. The prominent bands at ∼55 kDa and ∼25 kDa are IgG heavy (IgG-H) and IgG light (IgG-L) chains, respectively. The nsp1β band is observed just above the IgG-L band in the nsp1β lane only. The four bands specific to the nsp1β lane are indicated by asterisks (*). The relative mobility of molecular mass markers (in kDa) is shown on the left.