Fig. 6.

PCBP1 and PCBP2 are not required for the translation of genomic RNA. BHK-21 cells treated with siRNAs (20 nM) shown on top of each lane for 72 h before being electroporated with pFL12Pol⁻ full-length RNA. At 24 h postelectroporation, cells were harvested and PRRSV nsp1α and nsp1β proteins were detected by immunoblotting. The level of β-actin was measured to ensure equal protein loading. The left lane shows protein from BHK-21 cells that were neither siRNA treated nor electroporated with pFL12Pol⁻ RNA.