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Analysis of the protein recognized by neutralizing MAb KH-1. 293T cells were either mock transfected (panels 1) or transfected with the pcDNA3.1(+)-BgQ1 vector (panels 2), pCAGGS-BgQ2-myc (panels 4), or pcDNA3.1(+)-BgQ1 with pCAGGS-BgQ2-myc (panels 3). The cells were harvested at 72 h posttransfection and then fixed and stained for IFA with MAb KH-1 (A) or MAb BgQ1-202 (B) and with c-myc antibody for gQ2 and Hoechst 33258 dye.
