Fig. 8.

Schematic diagram of HHV-6B gQ1 mutants with various amino-terminal truncations and their reactivities to MAb KH-1. 293T cells were cotransfected with a plasmid expressing gQ1 or individual gQ1 truncation mutants and a plasmid expressing gQ2. The cells were stained for IFA at 72 h posttransfection with MAb KH-1 or BgQ1-202. The reactivities of KH-1 and BgQ1-202 for each gQ1 variant are shown at the right. The black box indicates the predicted signal sequence (aa 1 to 25) on HHV-6B gQ1. The N-terminal deletion mutants without the signal sequence (aa 1 to 25) containing a 6×His tag at the C terminus were fused to the IL-2 signal sequence instead of that of gQ1.