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. 2011 Dec;85(24):13373–13383. doi: 10.1128/JVI.05423-11

Fig. 5.

Fig. 5.

Secretion of HBV particles in the presence of trafficking inhibitors. HepG2 2.2.15 cells were grown for 6 h in complete medium in the presence of BAF (100 nM), BFA (2.5 μM), CQ (50 μM), or WO (1 μM) or with no treatment. (A) The viral DNA secreted from an equal number of cells was purified and quantified by real-time PCR. The results were expressed in genome equivalents (GEq) as percentages of the values for untreated samples. Data represent the means and SDs of duplicates from two independent experiments. (B) The amount of HBsAg secreted from an equal number of cells was quantified by ELISA. Data represent the means and SDs of triplicates from two independent experiments and are presented as percentages of the values for untreated samples. The cutoff values varied from 0.082 to 0.096.