Fig. 2.

Replication kinetics and plaque morphology of the YE102-VC1 virus in comparison to those of HSV-1(F)-YE102. (A) Representative viral plaques of HSV-1(F)-YE102 and HSV-1(F)-YE102-VC1 on Vero cells at 48 hpi. Viral plaques were visualized by phase-contrast microscopy after immunostaining with anti-HSV-1 polyclonal antibody. (B) One-step replication kinetics of HSV-1(F)-YE102 and HSV-1(F)-YE102-VC1 at an MOI of 0.2 on Vero cells. The x axis shows hours postinfection, and the y axis shows PFU/ml. All experiments were performed in triplicate. Error bars represent standard errors of the means.