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Transmission immunogold electron microscopy to detect gK and gB on virion particles. (A) HSV-1(F)-YE102 virions stained with anti-gK (anti-V5) antibody; (B) YE102-VC1 stained with anti-gK (anti-V5) antibody; (C) gK-V5-TEV stained with anti-gK (anti-V5) antibody.