Fig. 5.

Assessment of the efficiencies of gK-null and gKΔ31–68 virus entry into Vero cells. (A) The relative efficiency of entry is shown as the percentage of cells expressing ICP4 at 12 hpi. Mean values and standard deviations for three independent experiments were calculated as shown. (B) Representative kinetics of virus HSV-1(F), gK-null, and gKΔ31-68 entry into Vero cells. Vero cell monolayers were infected with an MOI of 1. At different times postentry at 34°C (10, 20, 30, and 60 min), viruses that had not entered yet were inactivated with low pH, and the kinetics of virus entry were determined by monitoring ICP4 expression at 12 hpi using flow cytometry. For each sample, the percent ICP4-positive cells in a population of 5,000 Vero cells was measured.