Fig. 6.

Construction and characterization of recombinant viruses containing TEV modifications. (A) Representative plaque morphology of the gDΔTEV and gK-V5-TEV viruses in comparison to those of their parental HSV-1(F)-YK608 virus at 48 hpi. Viral plaques were visualized after immunohistochemical staining using phase-contrast microscopy and by fluorescence microscopy. (B) Replication kinetics of HSV-1(F) YK608, gDΔTEV, and gK-V5-TEV viruses on Vero cells infected with an MOI of 0.2. The x axis shows hours postinfection, and the y axis shows PFU/ml. All experiments were performed in triplicate. Error bars represent standard errors of the means.