Fig. 8.

The effect of TEV protease treatment on virion infectivity. gDΔTEV (A) or gK-V5-TEV (B) virus obtained from supernatants of infected cells was treated with PBS, AcTEV protease (PBS + E), or SDS-inactivated AcTEV protease [PBS + INACT.E (CP)], and the numbers of resultant PFU were determined. Similarly, the relative kinetics of viral entry were determined for gDΔTEV (C) or gK-V5-TEV (D) in the presence (PBS + E) or absence (PBS) of the AcTEV protease. These experiments were done in triplicate, and error bars represent standard errors of the means. Asterisks in panel B designate statistically significant differences as calculated by one-way analysis of variance (ANOVA) (P < 0.05).