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. 2011 Dec;85(24):13069–13076. doi: 10.1128/JVI.00829-11

Fig. 1.

Fig. 1.

Hypoxia upregulates gene expression of LCMV MX. (A) DMOG enhances expression of viral genes. HeLa-MX cells were incubated for 24 h with or without 1 mM DMOG (a hypoxia-mimicking agent) in normoxia. Expression of LCMV genes was determined by quantitative RT-PCR. Values represent means from two separate experiments, each done in triplicates, with error bars denoting standard deviations. *, P < 0.05; ***, P < 0.001 (DMOG versus no DMOG). (B) HeLa-MX cells were cultured under normoxic or hypoxic conditions for 48 h and analyzed by quantitative RT-PCR. For each gene, the fold induction in hypoxia was determined in comparison with the normoxic control. Values represent means from three separate experiments, each done in quadruplicates. Error bars denote the standard deviations. *, P < 0.05; ***, P < 0.001 (hypoxia versus normoxia). (C) HeLa-Arm cells were cultured under normoxic or hypoxic conditions for 48 h, subjected to quantitative RT-PCR, and evaluated as for panel B. *, P < 0.05; **, P < 0.01; ***, P < 0.001.