Fig. 5.

Effect of HIF-α subunit suppression on LCMV MX gene expression under hypoxia. (A) HeLa-MX cells were transfected with the specific HIF-1α, HIF-2α, and control siRNAs and after 24 h were transferred to hypoxia for additional 48 h. Total RNA was then extracted and subjected to real-time RT-PCR analysis to determine expression of viral genes. Fold induction was determined in comparison with the values from cells transfected with control siRNA. All values represent means from triplicate determinations in one representative experiment out of two, with error bars denoting standard deviations. (B) HeLa-MX cells transfected with siRNA were analyzed by quantitative RT-PCR to determine the effect of HIF-1α and HIF-2α suppression on their transcriptional targets. Data were analyzed and illustrated as described above.