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VACV E3 colocalizes with SUMO1 in viral factories. (A) SUMO1 is recruited to viral DNA replication sites. Uninfected cells or MCF-7 cells infected with VACV for 6 h were fixed and stained with DAPI (4′,6-diamidino-2-phenylindole) and anti-SUMO1 and anti-vaccinia virus antibodies. (B) SUMO1 colocalizes with E3 in viral factories. MCF-7 cells infected with VACV for 6 h were fixed and stained with DAPI and anti-SUMO1 and anti-E3 antibodies. The images were analyzed by confocal microscopy.
