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Schematic for synthesis of mRNA and replication of the viral RNA of all eight segments (consensus sequence shown) from ISAV (18, 28). The mRNA is defined by a 5′ catabolite gene activator protein (CAP) structure that is derived from cellular mRNA and a 3′ poly(A) tail. In contrast, the cRNA from which copies of the genomic vRNA are made does not have these modifications.
