Fig. 6.
Confirmation of iridoptin-induced apoptosis in boll weevil (AG) cells by TUNEL assay. AG cells were grown on glass slides in CellStar six-well plates (5.6 × 103 cells per well in 2.5 ml of medium). Cells were treated as described in Materials and Methods and viewed by phase-contrast microscopy. Brown staining of the nuclei indicated positive DAB signal. Assays were performed in duplicate. Treatments were as follows: 10 μg/ml iridoptin (A), 20 μg/ml iridoptin (B), 4 μg/ml actinomycin D (C), and mock treatment (D). Magnification, ×200. Insets show detail of nuclear DAB signal (magnification, ×400).