Fig. 1.
Virological analysis of brain stems and livers. Control or Stat1−/− mice were inoculated on the cornea with 2 × 106 PFU/eye of HSV-1 WT or HSV-1 Δvhs. At the indicated time postinfection, brain stems (A) and livers (B) were harvested and mechanically disrupted for a plaque assay (n ≥ 8). Titers are presented as PFU/tissue and are the average of results for at least 8 animals. The dotted line indicates threshold of detection (LOD). Where average values fall below the LOD and error bars are shown, virus was detected in some samples and not in others. Where no error bars are shown, virus in all samples was below the LOD. Asterisks indicate ranges of statistical significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001) for differences between bracketed bars. All bars on days 3 and 5 showed statistically significant differences from each other, with the exception of results for WT-infected control mice and Δvhs-infected Stat1−/− mice, which were not significantly different.