Fig. 1.
Analysis of satsiRNA association with AGO-containing complexes. (A) SD-CMV infection symptoms in wild-type Arabidopsis (Col-0) and 6-Myc-AGO1 ago1-27 (6myc-AGO1) transgenic Arabidopsis plants. Photographs were taken at 10 dpi. (B) AGO-containing complexes were immunopurified from total protein extracted from SD-CMV-infected Col-0 and 6myc-AGO1 plants by using specific antibodies as indicated. Total protein extracts incubated with protein A were used as negative controls (−). (C) Small RNA detection of copurified satsiRNAs and miRNAs. Small RNAs were extracted from immunoprecipitates of AGO-containing complexes and protein A controls as indicated at the top. Membranes were hybridized with 32P-labeled specific oligodeoxynucleotide probes for each satsiRNA and miR-159 and miR-390a. U6 RNA hybridization is shown as an input sample control.