Fig. 4.
Analysis of the effect of asatsiR-12 on regulating expression of GFP-3′UTR2. (A and C) and GFP-3′UTR3 (B and D) in wild-type N. benthamiana plants (A and B) and RDR6i plants (C and D). Photographs were taken under UV light at 2, 3, and 4 dpi. The expression of asatsiR-12 and the accumulation of GFP-3′UTR2 and GFP-3′UT3 RNAs, 5′ cleavage products, and 3′UTR2- and 3′UTR3-derived siRNAs (siR-3′UTR2 and siR-3′UTR3), as well as the expression of GFP, were detected as described in the legend to Fig. 3.