Fig. 6.
Analysis of the inhibitory effect of SD-CMV 2b on asatsiR-12-mediated cleavage of GFP-3′UTR1. (A) Coexpression of GFP-3′UTR1 with asatsiR-12 (siR-12) or a vector control (V) in the absence or presence of SD-CMV 2b protein in wild-type N. benthamiana plants (left panel) and RDR6i plants (right panel). GFP fluorescence was photographed under UV light at 4 dpi. (B) Expression of asatsiR-12 and accumulation of SD-CMV 2b and GFP-3′UTR1 RNA and 5′ cleavage products, as well as expression of GFP, were detected as described in the legend to Fig. 3B.